Research Activities

Agricultural Biotechnology Research Institute (ABRI) is engaged in research work for the improvement of agricultural crops using latest tools of biotechnology in collaboration with other crop institutes of Ayub Agricultural Research Institute, Faisalabad. Application of biotechnological tools to facilitate plant breeding is on top priority in activities of ABRI. Due to established worth of genetic engineering in crop improvement, now considerable emphasis is being given to transgenic research for the development of better crop varieties with desirable combination of genes. Presently research work is being carried out on following crops in various desciplines of biotechnology indicated below:

On-Going

Tissue Culture

  • Sugarcane
  • Wheat
  • Rice
  • Brassica
  • Stevia
  • Banana
  • Potato

Molecular Breeding

  • Wheat
  • Rice
  • Cotton
  • Sunflower
  • Chickpea
  • Mungbean
  • Tomato

Genetic Engineering

  • Brassica
  • Cotton
  • Sugarcane
  • Wheat
  • Tobacco

Diagnostics

  • GMO detection
  • Bt identification & Quantification
  • Basmati Rice
  • Purity in Rice
  • Hybrids

Soil Bacteriology

  • Wheat
  • Rice
  • Cotton
  • Brassica
  • Mungbean
  • Chickpea
  • Fodders

Following are the experiment wise salient research activities being executed at ABRI:

  • Codon optimization, characterization and expression analysis of genes for crop improvement
  • Detection and quantification of Bt toxin in various parts of cotton plant
  • Pollen tube pathway mediated genetic transformation for herbicide tolerant gene in cotton
  • Genetic transformation and expression analysis for drought tolerance in sugarcane
  • Integration of modified Glyphosate Tolerance gene (EPSPS) in sugarcane and wheat plants
  • Application of genetic engineering to improve oil quality in Brassica juncea
  • Application of genetic engineering to improve harvest index in Brassica juncea
  • Testing and identification of GM elements ingenetically modified crops
  • Identification of redrot resistant sugarcane genotypes through linked molecular markers
  • Molecular Marker assisted selection of drought tolerant wheat genotypes
  • Multiple rust resistance gene pyramiding in wheat using linked DNA markers
  • Evaluation of genetic diversity in rice somalclones
  • Propagation of olive plants through stem cuttings
  • Exploitation of somaclonal variation in Wheat for yield improvement
  • Development of rust resistant somaclones of Wheat genotypes Seher-06, Faisalabad-08, Galaxy-13 and Ujala-16
  • Maintenance & utilization of Wheat genepool for crossing & use in tissue culture
  • Somaclonal variation studies in Wheat regenerations (R1 – R6)
  • Preliminary Wheat yield trials (A-trials)
  • Regular Wheat yield trials (B-trials)
  • Micro-plot Wheat yield trials (Irrigated &Rainfed)
  • Development of drought tolerant Wheat plants through tissue culture
  • Effect of different media for callus induction and optimization of proficient regeneration system in Rice
  • Screening of Wheat advance lines of ABRI against leaf, strip and stem rusts
  • Screening of advanced Wheat material against rusts at SARS, Kaghan
  • Screening of Sugarcane somaclones/lines against Red Rot under field conditions
  • Micropropagation of Potato variety SH-5 and their screenings through ELISA
  • Use of Calliclonal variation for Sugarcane improvement
  • Introducing salt tolerance in Wheat through the induction of somaclonal variation

Completed

  • Optimization of regeneration protocol for lentil
  • Control of Bacterial Leaf Blight (BLB) in Rice through management and resistant varieties
  • Identification of rust and karnal bunt resistance genes in advance lines of wheat
  • Identification of DNA markers for resistance against Burewala virus disease in upland Cotton
  • Validation of DNA markers for sex determination in jojoba
  • Identification of resistant genotypes for ascochyta blight in chickpea  for marker-assisted selection
  • Application of molecular markers for the detection of resistant genotypes against tomato mosaic virus in tomato
  • Micropragation of olive through shoot tip culture
  • In-vitro studies in Sugarcane for inducing salt tolerance
  • Isolation and screening of Agrobacterium species from plants and soil