Achievements

Following are the achievements of the Institute:

Vision of the Agri. Biotechnology Research Institute Faisalabad is to provide support services to all other Research Institutes in Agriculture Research System (AARI) regarding latest biotechnological techniques for the development of new crop varieties and diagnostic services based on protein (ELISA) and nucleic acids (DNA finger printing). During 2001-2002 to 2004-2005 an ADP project “Seed propagation of sugarcane and potato through tissue culture” was implemented and 93800 tissue culture plantlets (Target=78000) and 611640 kg disease free seed of sugarcane (Target=60000kg) was produced. Another ADP project “Up-scaling of Agri. Biotechnology Research Institute, AARI, Faisalabad” was implemented from 2005-06 to 2010-11 and all targets were achieved including establishment of genomics laboratories. Furthermore, DNA fingerprinting of 71 rice, 60 wheat, 80 sugarcane, 95 sugarcane and 48 sorghum genotypes was accomplished and results were conveyed to respective department for utilization of these finding for identification of parents in their breeding programs. About 2290 doubled haploids of wheat were produced to cut short time period required for Wheat varietal development and were handed over to Wheat Research Institute, AARI, Faisalabad. More than 3000 somaclones of wheat and sugarcane were produced for enhancement of genetic variation and enrichment of germplasm and about 44580 disease free mericlones were produced. One more ADP project “Establishment of Lab for the testing of Biotech crops” was completed (2012-13 to 2014-15) achieving all the targets set in the PC-I and established state of the art GMO testing laboratory. In this project,3530 samples (cotton=2720, canola=350, maize=460) were assayed for the presence/absence of biotech traits. In cotton maximum samples were positive for Bt trait whereas, no sample of maize and canola was found positive for any transgenic element/trait which shows that no GMO rice/maize is being cultivated in the surveyed areas of Punjab. During three years of the project implementation, 655 samples of all the 131 entries (candidate varieties and genotypes) of NCV trial were analyzed using lateral flow strip test, PCR and ELISA for detection, identification and quantification of gene/gene products which paved the way for development of Bt cotton varieties. These services were provided to all the local research stations of Cotton Research Institute, AARI Faisalabad located at Multan, Vehari, Bahawalpur and Sahiwal. Rice Research Institute, Kala Shah Kaku collected the samples of imported hybrid rice on a public complaint about the presence of GMO, these samples were tested no GMO element was found present in these samples. The detection, identification and quantification of GMOs services were extended to private seed companies on charge basis. 93 personnel belonging to public and private sector were trained in the field of “the testing of biotech crops” during three years. In addition to all this following achievements were also made by this institute:

  • One high yielding and rust resistant wheat variety Ufaq-2002 was developed through somaclonal variation and approved for general cultivation in the irrigated areas of Punjab province. Among other genotypes developed by this institute, V05BT014 which was contributed to national level testing trials (NUWYT) stood 2nd in Punjab during 2011-12, V-08BT016 performed better in provincial testing trail (MWYT) as compared to check variety. Newly developed variety V15BT022 and V14BT004 performed better than check variety at provincial level during the year 2016-17
  • Sugarcane red rot resistant variety HSF-242 was evolved through somaclonal variation in collaboration with Sugarcane Research Institute and was approved for general cultivation during 2007
  • The Accreditation of GMO lab UNDER ISO-17025 is in its final stages. This will improve authenticity of test results of the GMO testing laboratory and bring it to international standards
  • Optimization of DNA isolation, RAPD, SSR, EST-SSR, ISSR, SCAR, CAPS etc. protocols for rice, wheat, sugarcane, cotton, brassica, groundnut, olive, fungus and other field crops was accomplished
  • Screening of Basmati rice material for the status of genes conferring resistance against BLB was carried out. A total of 4452  samples obtained from Rice Research Institute, Kala Shah Kaku and Emkay Seeds, Farooqabad were assayed for presence of resistance genes for BLB strains (Xa4, Xa5, Xa7, Xa13 & Xa21) using gene-based/linked DNA markers
  • 80 wheat genotypes from different research institutes and Micro trial were tested at molecular level using published molecular/DNA markers linked to various Leaf, Yellow and Stem rust resistant genes for their pyramiding in single variety
  • Synthetic nucleotide sequence of insect resistant Cry1Ac&Cry2Ab genes submitted to GenBank & accession no. was allotted i.e. KX622797 & KX622798, respectively
  • Synthetic IR genes transformed into model system and confirmed
  • Characterization studies of modified GT gene in model system
  • Since year 2009, 7309 samples  of Cotton, Rice, Maize, Brassica etc. from public and private sector were tested, identified and quantified using strip, PCR and ELISA techniques
  • DNA markers for hybrid identification in case of sugarcane were identified for their use in breeding programs. Crossing scheme/parental combinations were suggested to Sugarcane Research Institute Faisalabad and Udawalawe, SriLanka to undertake hybridization work
  • DNA fingerprinting and Genetic diversity studies of Wheat (84), Rice (70), Sugarcane (90), Cotton (95), Groundnut (78), Flex (30), Raya (31), Sorghum (48), Olive (32) genotypes / varieties was accomplished
  • DNA key for the identification of olive varieties was developed and services provided to BARI, Chakwal for propagation of certified olive nursery to enhance olive production
  • Groundnut varieties resistant to late Leaf Spot disease were identified through Molecular markers for their use in breeding for the development of late leaf blight groundnut varieties were conveyed to BARI Chakwal
  • Methodology for the identification of basmati rice at DNA level was developed and services are being provided to Rice Research Institute, Kala Shah Kaku (export of Basmati rice to Europe in linked with DNA testing)
  • 35026 microbial inoculum packets for the improvement of NPK were produced and supplied to the farmers during last five years.Rhizobialinocula increased crop yield by 5-35% depending upon crop specific conditions. Use of PGPR as biofertilizers enhanced cereals yields by 5-15% in field and pot trials conducted at various locations
  • Phosphobacterium (PSM) increased P-use efficiency to noticeable amount and yielded significantly higher grains
  • About 500 internship, 40 M.Sc. and 4 Ph.D. scholars were trained in the field of biotechnological tools during last three years